PA5-96512

antibody from Invitrogen Antibodies

Targeting: CSF2 GM-CSF, GMCSF

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96512

Provider

Invitrogen Antibodies

Product name

GM-CSF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: Mouse intestine Immunogen sequence: ARSPSPSTQP WEHVNAI

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

2.52 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GM-CSF in extracts of various cell lines. Samples were incubated with GM-CSF Polyclonal antibody (Product # PA5-96512) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GM-CSF in C6 cells. Samples were incubated with GM-CSF Polyclonal antibody (Product # PA5-96512) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GM-CSF was performed in L929 cells using GM-CSF Polyclonal Antibody (Product # PA5-96512) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GM-CSF was performed in U-2 OS cells using GM-CSF Polyclonal Antibody (Product # PA5-96512) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GM-CSF in L929 cells. Samples were incubated with GM-CSF Polyclonal antibody (Product # PA5-96512) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GM-CSF in U-2 OS cells. Samples were incubated with GM-CSF Polyclonal antibody (Product # PA5-96512) using a dilution of 1:100. Blue: DAPI for nuclear staining.