Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-215-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human GM-CSF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human GM-CSF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) G-CSF, rhIL-1 alpha , rhIL-1 beta , rhIL-2, rhIL-3, rhIL-4, rhIL-6, rhTNF-alpha or rhTNF-beta is observed. Neutralizes the biological activity of both rhGM-CSF and natural human GM-CSF. It will not neutralize the biological activity of recombinant mouse GM-CSF.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P04141
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
Solanilla A, Déchanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
Blood 2000 Jun 15;95(12):3758-64
Blood 2000 Jun 15;95(12):3758-64
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Human GM-CSF Antibody. Recombinant Human GM-CSF (Catalog # 215-GM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM-CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Human GM-CSF Polyclonal Antibody (Catalog # AB-215-NA). The ND50 is typically 1-6 µg/mL.