Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS4326 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- MEF2D (phospho S444) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human MEF2D around the phosphorylation site of Serine 444.
- Description
- The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C, and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-MEF2D (S444) pAb at 1:500 dilutionLane1:HEK293T cell lysateLane2:Raw264.7 cell lysateLane3:PC12 cell lysate