Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB8900 - Provider product page
- Provider
- R&D Systems
- Product name
- Human ITFG1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ITFG1 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8TB96
- Isotype
- IgG
- Antibody clone number
- 936234
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human ITFG1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, human brain (cerebellum) tissue, and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB8900) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ITFG1 at approximately 95-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.