Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-55311 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AGMAT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLGTVNPSTG ALPFQSLMVA DLGDVNVNLY NLQDSCRRIQ EAYEKIVAAG CIPLTLGGDH TITYPILQAM AKKHGPVGLL HVDAHTDTTD KALGEKLYHG Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 80%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AGMAT in control (vector only transfected HEK293T lysate) and AGMAT over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a AGMAT Polyclonal Antibody (Product # PA5-55311).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AGMAT in human cell lines Caco-2 and U-251MG using a AGMAT Polyclonal Antibody (Product # PA5-55311). Corresponding AGMAT RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AGMAT in human kidney and lymph node tissues using AGMAT Polyclonal Antibody (Product # PA5-55311). Corresponding AGMAT RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AGMAT in human colon using AGMAT Polyclonal Antibody (Product # PA5-55311) shows moderate granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AGMAT in human duodenum using AGMAT Polyclonal Antibody (Product # PA5-55311) shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AGMAT in human lymph node using AGMAT Polyclonal Antibody (Product # PA5-55311) shows very weak cytoplasmic positivity in lymphoid cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of AGMAT in human kidney using AGMAT Polyclonal Antibody (Product # PA5-55311) shows strong granular cytoplasmic positivity in cells in tubules.