PA5-53468

antibody from Invitrogen Antibodies

Targeting: SLC10A6 SOAT

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53468

Provider

Invitrogen Antibodies

Product name

SLC10A6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KRRLKNKHGK KNSGCTEVCH TRKSTSSRET NAFLEVNEEG AITPGPPGPM DCHRALEPVG HITSCE Highest antigen sequence identity to the following orthologs: Mouse - 44%, Rat - 33%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cholesterol sulfate alleviates ulcerative colitis by promoting cholesterol biosynthesis in colonic epithelial cells.

Xu D, Ma R, Ju Y, Song X, Niu B, Hong W, Wang R, Yang Q, Zhao Z, Zhang Y, Zheng Y, Bai Q, Lv M, Sun N, Li X
Nature communications 2022 Jul 30;13(1):4428

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 CS promotes cholesterol biosynthesis by binding to NPC2 and activating SREBP2. a Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis for the up-regulated differentially expressed genes in CS-treated HT-29 cells compared with control HT-29 cells (treated with DMSO). (Fold change >=1.5, P < 0.05). b Heatmaps for the steroid biosynthesis genes in the upregulated genes group in CS-treated HT-29 cells compared with control HT-29 cells (treated with DMSO). c RT-qPCR analysis of HMGCS1 , DHCR7 , FDFT1 and CYP51A1 mRNA levels in HT-29 cells, LOVO cells, SW480 cells, HCT116 cells, SW1116 cells, and NCM460 cells treated with CS (50 muM) or DMSO for 24 h ( n = 2 independent culture wells, two-tailed Student's t- test). d The cholesterol concentration of HT-29 cells treated with CS (50 muM) for 6, 12, 24, and 48 h measured by the Amplex(r) Red Cholesterol Assay Kit ( n = 4 biologically independent samples, one-way ANOVA with Sidak's multiple comparison test). e Filipin III staining in HT-29 and SULT2B1 -KO HT-29 cells in the presence or absence of CS (50 muM) ( n = 4 fields/group, one-way ANOVA with Sidak's multiple comparison test). f Cell viability of HT-29 and SULT2B1 -KO cells treated with 25 muM or 50 muM CS ( n = 4, 3, 3 biologically independent samples/group, respectively, one-way ANOVA with Sidak's multiple comparison test). g Transcriptional profiles of SLC family members in colonic tissues from Sult2b1 f/f and Sult2b1 IEC mice challenged with 2.5% DSS ( n =