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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [6]
- Flow cytometry [2]
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- Product number
- TA502247 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502247, RRID:AB_11125025
- Product name
- GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3C6 (formerly 3C6)
- Antibody type
- Monoclonal
- Description
- GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3C6 (formerly 3C6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GPHN
- Isotype
- IgG
- Antibody clone number
- OTI3C6
- Vial size
- 100 µl
- Concentration
- 0.83 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GPHN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502247)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502247), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-GPHN antibody(TA502247),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
