TA502312

antibody from OriGene

Targeting: GPHN KIAA1385

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502312

Provider

OriGene

Proper citation

OriGene Cat#TA502312, RRID:AB_11125592

Product name

GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3A6 (formerly 3A6)

Antibody type

Monoclonal

Description

GPHN (Gephyrin) mouse monoclonal antibody, clone OTI3A6 (formerly 3A6)

Host

Mouse

Conjugate

Unconjugated

Epitope

GPHN

Isotype

IgG

Antibody clone number

OTI3A6

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GPHN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GPHN (RC205986, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPHN.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-GPHN antibody(TA502312),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GPHN antibody(TA502312), and then analyzed by flow cytometry.

Validation comment

FC