Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARG53997 - Provider product page
- Provider
- Arigo
- Product name
- anti-HP1 gamma antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human HP1-gamma protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5G10-F7-A12
- Vial size
- 100 µl
- Concentration
- 0.75 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HP1-gamma in HeLa, 3T3, C6, COS7 and CHO-K1 cell lysates stained with HP1 gamma antibody [5G10-F7-A12] (ARG53997) (diluted in 1: 1000). Predicted band size: 22KDa. Observed band size: 22KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with HP1 gamma antibody [5G10-F7-A12] (ARG53997) (diluted in 1:200).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: HeLa cell lysates immunoprecipitated and stained by HP1 gamma antibody [5G10-F7-A12] (ARG53997).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Prostate Cancer stained with HP1 gamma antibody [5G10-F7-A12] (ARG53997) (diluted in 1/200). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).