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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-17210 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-HP1 gamma (Ser83) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with HP1-alpha or HP1-beta proteins.
- Reactivity
- Human, Mouse, Rat, Bovine, Drosophila
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 484 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-HP1-gamma pSer83 in whole cell extracts from HeLa cells, untreated and treated for 1 h with Forskolin (30 uM) and IBMX (0.5 mM) using Phospho-HP1-gamma pSer83 polyclonal antibody (Product # PA5-17210) (upper panel) or a HP1-gamma polyclonal antibody (lower panel).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-HP1-gamma pSer83 in whole cell extracts from HeLa cells, untreated and treated for 1 h with Forskolin (30 uM) and IBMX (0.5 mM) using Phospho-HP1-gamma pSer83 polyclonal antibody (Product # PA5-17210) (upper panel) or a HP1-gamma polyclonal antibody (lower panel).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-HP1-gamma pSer83 in HeLa cells, forskolin and IBMX-treated (center), using a Phospho-HP1-gamma pSer83 polyclonal antibody (Product # PA5-17210) (green). Actin filaments are labeled with a fluorescent red phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-HP1-gamma pSer83 in HeLa cells using a Phospho-HP1-gamma pSer83 polyclonal antibody (Product # PA5-17210) (green). Actin filaments are labeled with a fluorescent red phalloidin.
