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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-30954 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HP1 gamma Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (90%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung.
Lüdtke TH, Wojahn I, Kleppa MJ, Schierstaedt J, Christoffels VM, Künzler P, Kispert A
Respiratory research 2021 Mar 17;22(1):85
Respiratory research 2021 Mar 17;22(1):85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HP1 gamma Polyclonal Antibody (Product # PA5-30954). Sample (30 µg of whole cell lysate). Lane A: HepG2. 12% SDS PAGE. HP1 gamma Polyclonal Antibody (Product # PA5-30954) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HP1 gamma in paraformaldehyde-fixed HeLa cells using a HP1 gamma polyclonal antibody (Product # PA5-30954) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HP1 gamma Polyclonal Antibody detects HP1 gamma protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HP1 gamma Polyclonal Antibody (Product # PA5-30954) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HP1 gamma Polyclonal Antibody detects HP1 gamma protein at nucleus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HP1 gamma Polyclonal Antibody (Product # PA5-30954) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using HP1 gamma (Product # PA5-30954) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HP1 gamma Polyclonal Antibody immunoprecipitates HP1 gamma protein in IP experiments. IP Sample: HeLa whole cell lysate/extract. A. 40 µg HeLa whole cell lysate/extract. B. Control with 2 µg of preimmune rabbit IgG. C. Immunoprecipitation of HP1 gamma protein by 2 µg of HP1 gamma Polyclonal Antibody (Product # PA5-30954). 15% SDS-PAGE. The immunoprecipitated HP1 gamma protein was detected by HP1 gamma Polyclonal Antibody (Product # PA5-30954) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Interaction candidates are coexpressed with TBX2 in the pulmonary mesenchyme and interact in HEK293 cells. a Co-immunofluorescence analysis of candidate interaction partners (red) and TBX2 (green) on frontal sections of the right lung of E14.5 Tbx2 cre/ + embryos. Antigens are color-coded and nuclei were counterstained with DAPI (blue). Insets or selected regions in overview images are magnified in rows 2,4 and 6. b In situ proximity ligation assay of TBX2 and candidate interaction partners on 10 um frontal sections of E14.5 wildtype and Tbx2 cre/fl mutant lungs. Direct interaction is visualized by small red fluorescent dots. Larger more diffuse orange stains are due to auto-fluorescence of blood cells. Nuclei are counterstained with DAPI (blue). c Western blot analysis of co-immunoprecipitation experiments for verification of TBX2 interaction with candidate proteins on 10% SDS polyacrylamide gels. Detection was performed with an anti-TBX2 primary antibody and developed with chemoluminescence-IHC. Arrows indicate TBX2 bands. Lanes were loaded as follows: No antibody: IP without specific antibody resembling negative IP-control; 5% input: 5% of crude cell extract before precipitation; empty: no protein loaded; IP: co-immunoprecipitate with antibody for specific candidate. Expected molecular weight for TBX2.HA approx. 76.2 kDa
