PA5-98952

BARHL1 antibody from Invitrogen Antibodies

Western blot

Supportive data in Antibodypedia

ELISA

Recommended by provider

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for PA5-98952

Antibody Data

Product number

PA5-98952

Provider

Invitrogen Antibodies

Product name

BARHL1 Polyclonal Antibody

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: SQVSQNCHNG SYEISVLMMG NSAFAEPLKN LEDAVNEGLE IVRGRLQNAG LNVTVNATFM YSDGLIHNSG DCRSSTCEGL DLLRKISNAQ RMGCVLIGPS CTYSTFQMYL

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

4.27 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BARHL1 using a BARHL1 Polyclonal antibody (Product # PA5-98952) at a concentration of 3.4 µg/mL. Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 36 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of BARHL1 using a BARHL1 Polyclonal antibody (Product # PA5-98952) at a concentration of 3.4 µg/mL. Positive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 36 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of BARHL1 in paraffin embedded human testis tissue using a BARHL1 polyclonal antibody (Product # PA5-98952) at a dilution of 1:400. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.