Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [7]
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Validation data
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- Product number
- PA5-54036 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HEMGN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VISVIQDHPF KMYQDMAKRE DLAPKMCQEA AVPKILPCPT SEDTADLAGC SLQAYPKPDV PKGYILDTDQ NPAEPEEYNE TDQGI Highest antigen sequence identity to the following orthologs: Mouse - 41%, Rat - 34%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot-Specific
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HEMGN in control (vector only transfected HEK293T lysate) and HEMGN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a HEMGN Polyclonal Antibody (Product # PA5-54036).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HEMGN in human kidney using HEMGN Polyclonal Antibody (Product # PA5-54036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HEMGN in human testis using HEMGN Polyclonal Antibody (Product # PA5-54036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HEMGN in human bone marrow shows strong cytoplasmic and nuclear positivity in a subset of bone marrow poietic cells. Samples were probed using a HEMGN Polyclonal Antibody (Product # PA5-54036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HEMGN in human colon using HEMGN Polyclonal Antibody (Product # PA5-54036).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HEMGN in human bone marrow and cerebral cortex tissues using HEMGN Polyclonal Antibody (Product # PA5-54036). Corresponding HEMGN RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HEMGN in human bone marrow, colon, kidney and testis using HEMGN Polyclonal Antibody (Product # PA5-54036) (A) shows similar protein distribution across tissues to an independent HEMGN Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of HEMGN in human cerebral cortex using HEMGN Polyclonal Antibody (Product # PA5-54036) shows low expression as expected.