Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-36210 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXJ1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 45 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Distinct Epithelial Cell Profiles in Normal Versus Induced-Congenital Diaphragmatic Hernia Fetal Lungs.
Gonçalves AN, Correia-Pinto J, Nogueira-Silva C
Frontiers in pediatrics 2022;10:836591
Frontiers in pediatrics 2022;10:836591
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXJ1 in Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with FOXJ1 polyclonal antibody (Product # PA5-36210) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXJ1 using FOXJ1 polyclonal antibody (Product # PA5-36210) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXJ1 in Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with FOXJ1 polyclonal antibody (Product # PA5-36210) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FOXJ1 in paraffin-embedded human tonsil carcinoma using FOXJ1 polyclonal antibody (Product # PA5-36210) at a dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Spatiotemporal distribution of forkhead box J1 (FOXJ1) in normal and induced-congenital diaphragmatic hernia (CDH) rat model at pseudoglandular, canalicular, and saccular stages. Representative immunohistochemical evidence for FOXJ1 expression at (AA,B,a,b,g,h) embryonic day (E)17.5, (AC,D,c,d,i,j) E19.5, and (AE,F,e,f,k,l) E21.5 in normal (ctrl), nitrofen (NF), and CDH lungs, respectively. *Bronchiole; ☐ primordia of bronchiole; ¥ terminal bronchiole; [bronchioalveolar duct junction; & alveolar duct. Scale bar 50 muM. (B) Semi-quantitative analysis of FOXJ1 expression from pseudoglandular-to-saccular stages in ctrl, NF, and CDH lungs. Results are presented as mean +- SD. Symbols indicate the main effects and non-redundant interactions of T -test for independent samples. alpha p < 0.05. (C) Representative immunohistochemical evidence for the absence of multi-cilia on the plasma membrane of ciliated cells in bronchi at E21.5. Original magnification x600.