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LearnNBP1-78211
antibody from Novus Biologicals
Targeting: ANKHD1
FLJ10042, FLJ11979, FLJ14127, FLJ20288, KIAA1085, MASK, MASK1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP1-78211 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78211, RRID:AB_11041318
- Product name
- Rabbit Polyclonal ANKHD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ANKHD1 Antibody [NBP1-78211] - Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-ANKHD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ANKHD1 was also immunoprecipitated by rabbit anti-ANKHD1 antibody NBP1-78212, which recognizes a downstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ANKHD1 Antibody [NBP1-78211] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RAP1 antibody NBP2-04097 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RAP1 was also immunoprecipitated by rabbit anti-RAP1 antibodies NBP2-04098, which recognizes a downstream epitope, and NBP1-78211 which is generated against full-length recombinant human RAP1. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
