PA5-30427

antibody from Invitrogen Antibodies

Targeting: ANKHD1 FLJ10042, FLJ11979, FLJ14127, FLJ20288, KIAA1085, MASK, MASK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30427

Provider

Invitrogen Antibodies

Product name

ANKHD1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, HepG2, HCT116, Jurkat, Raji, NCI-H929. Predicted reactivity: Mouse (95%), Rat (95%), Dog (96%), Pig (96%), Chicken (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.35 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ANKHD1 Polyclonal Antibody (Product # PA5-30427). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. ANKHD1 antibody . ANKHD1 Polyclonal Antibody (Product # PA5-30427) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ANKHD1 Polyclonal Antibody detects ANKHD1 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract. B. 30 µg Raji whole cell lysate/extract. C. 30 µg NCI-H929 whole cell lysate/extract.7.5 % SDS-PAGE. ANKHD1 Polyclonal Antibody (Product # PA5-30427) dilution: 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ANKHD1 (Product # PA5-30427) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.