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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-21648 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NANS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, HepG2. Predicted reactivity: Mouse (97%), Rat (97%), Pig (97%), Chicken (88%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.21 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NANS Polyclonal Antibody (Product # PA5-21648). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. NANS Polyclonal Antibody . NANS Polyclonal Antibody (Product # PA5-21648) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NANS Polyclonal Antibody [N1C3] detects NANS protein at nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NANS protein stained by NANS Polyclonal Antibody [N1C3] (Product # PA5-21648) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human normal gastric epithelium (gland), using NANS (Product # PA5-21648) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
