PA5-50806

antibody from Invitrogen Antibodies

Targeting: TRIM14 KIAA0129

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-50806

Provider

Invitrogen Antibodies

Product name

TRIM14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The antibody detects endogenous levels of total TRIM14 protein.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.2 mg/mL

Storage

-20°C

References

Submitted references

Circular RNA circ_0048764 promotes the development of breast cancer by regulating microRNA-1296-5p/tripartite motif containing 14 axis.

Xie F, Xiong Y, Yan J, Wang L, Yan W
Bioengineered 2022 Feb;13(2):1963-1974

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Sequential ubiquitination of NLRP3 by RNF125 and Cbl-b limits inflammasome activation and endotoxemia.

Tang J, Tu S, Lin G, Guo H, Yan C, Liu Q, Huang L, Tang N, Xiao Y, Pope RM, Rajaram MVS, Amer AO, Ahmer BM, Gunn JS, Wozniak DJ, Tao L, Coppola V, Zhang L, Langdon WY, Torrelles JB, Lipkowitz S, Zhang J
The Journal of experimental medicine 2020 Apr 6;217(4)

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TRIM14 in paraffin embedded Human thyroid cancer tissue using TRIM14 Polyclonal Antibody (Product # PA5-50806) at a 1:40 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Circ_0048764/miR-1296-5p axis regulates BC progression by regulating the expression of TRIM14 (a)The potential binding site between TRIM14 mRNA 3' UTR and miR-1296-5p were predicted by targetscan database. (b) dual-luciferase reporter gene assay was used to examine the interaction of TRIM14 mRNA 3'UTR and miR-1296-5p in HEK-293 T cells. (c and d) the levels of TRIM14 mRNA and protein in SKBR3 cells transfected with pcDNA-NC, pcDNA-circ_0048764 and pcDNA-circ_0048764 + miR-1296-5p mimics, or MCF7 cells transfected with si-NC, si-circ_0048764-1 and si-circ_0048764-1 + miR-1296-5p inhibitors were detected by qRT-PCR and western blot assay, respectively. (e) the expression of TRIM14 mRNA in BC tissues and normal tissues was detected by qRT-PCR. (f and g) Pearson's correlation analysis was used to detect the correlation between TRIM14 mRNA expression and miR-1296-5p expression or circ_0048764 expression in BC tissues, respectively. ** P < 0.01, and *** P < 0.001.