Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF222 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Activin RIB/ALK-4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Activin RIB/ALK-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Activin RIA is observed and less than 2% cross-reactivity with rhActivin RIIA and rhActivin RIIB is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAA60556
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Homozygous deletion of the activin A receptor, type IB gene is associated with an aggressive cancer phenotype in pancreatic cancer.
Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary.
Togashi Y, Sakamoto H, Hayashi H, Terashima M, de Velasco MA, Fujita Y, Kodera Y, Sakai K, Tomida S, Kitano M, Ito A, Kudo M, Nishio K
Molecular cancer 2014 May 27;13:126
Molecular cancer 2014 May 27;13:126
Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct.
Maeshima A, Vaughn DA, Choi Y, Nigam SK
Developmental biology 2006 Jul 15;295(2):473-85
Developmental biology 2006 Jul 15;295(2):473-85
Follicle-restricted compartmentalization of transforming growth factor beta superfamily ligands in the feline ovary.
Bristol SK, Woodruff TK
Biology of reproduction 2004 Mar;70(3):846-59
Biology of reproduction 2004 Mar;70(3):846-59
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Activin RIB/ALK-4 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Activin RIB/ALK-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF222) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Activin RIB/ALK-4 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.