MA5-17209

antibody from Invitrogen Antibodies

Targeting: CDK7 CAK, CAK1, CDKN7, MO15, STK1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-17209

Provider

Invitrogen Antibodies

Product name

Anti-CDK7 Monoclonal Antibody (3E3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is HeLa cells.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3E3

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDK7 using anti-CDK7 monoclonal antibody (Product # MA5-17209) on (Lane 1) HeLa Cell lysate, (Lane 2) Jurket Cell lysate, (Lane 3) SK-N-MC Cell lysate and K562 Cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of CDK7 was achieved by transfecting A-431 with CDK7 specific siRNAs (Silencer® select Product # s2830). Western blot analysis (Fig. a) was performed using Whole cell extracts from the CDK7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CDK7 Monoclonal Antibody (3E3) (Product # MA5-17209, 0.4 ug/ml) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK7.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CDK7 Monoclonal Antibody (3E3) (Product # MA5-17209) and a 39 kDa band corresponding to CDK7 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4), HCT 116 (Lane 5) and K-562 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution), 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).