PA5-34791

antibody from Invitrogen Antibodies

Targeting: CDK7 CAK, CAK1, CDKN7, MO15, STK1

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-34791 - Provider product page
Provider: Invitrogen Antibodies
Product name: CDK7 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: 293T, A431, A375, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12. Predicted reactivity: Mouse (95%), Zebrafish (85%), Xenopus laevis (86%), Pig (97%), Rhesus Monkey (99%), Bovine (96%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.96 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CDK7 protein structure - PA5-34791 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK7 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate and C) 30 µg A375 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CDK7 polyclonal antibody (Product # PA5-34791) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK7 using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CDK7 polyclonal antibody (Product # PA5-34791) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using CDK7 Polyclonal Antibody (Product # PA5-34791). Various extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK7 Polyclonal Antibody (Product # PA5-34791) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CDK7 was achieved by transfecting A-431 with CDK7 specific siRNAs (Silencer® select Product # s2830). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CDK7 Polyclonal Antibody (Product # PA5-34791, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK7.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-CDK7 Polyclonal Antibody (Product # PA5-34791) and a 39 kDa band corresponding to CDK7 was observed across cell lines and tissue extract tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), NIH/3T3 (Lane 4), HeLa (Lane 5), Jurkat (Lane 6), HCT116 (Lane 7), K-562 (Lane 8) and tissue extracts of Mouse Testis (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).