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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-34791 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, A375, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12. Predicted reactivity: Mouse (95%), Zebrafish (85%), Xenopus laevis (86%), Pig (97%), Rhesus Monkey (99%), Bovine (96%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cell-Cycle Proteins Control Production of Neutrophil Extracellular Traps.
Amulic B, Knackstedt SL, Abu Abed U, Deigendesch N, Harbort CJ, Caffrey BE, Brinkmann V, Heppner FL, Hinds PW, Zychlinsky A
Developmental cell 2017 Nov 20;43(4):449-462.e5
Developmental cell 2017 Nov 20;43(4):449-462.e5
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK7 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate and C) 30 µg A375 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CDK7 polyclonal antibody (Product # PA5-34791) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK7 using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CDK7 polyclonal antibody (Product # PA5-34791) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CDK7 Polyclonal Antibody (Product # PA5-34791). Various extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK7 Polyclonal Antibody (Product # PA5-34791) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CDK7 was achieved by transfecting A-431 with CDK7 specific siRNAs (Silencer® select Product # s2830). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CDK7 Polyclonal Antibody (Product # PA5-34791, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK7.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CDK7 Polyclonal Antibody (Product # PA5-34791) and a 39 kDa band corresponding to CDK7 was observed across cell lines and tissue extract tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), NIH/3T3 (Lane 4), HeLa (Lane 5), Jurkat (Lane 6), HCT116 (Lane 7), K-562 (Lane 8) and tissue extracts of Mouse Testis (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDK7 (Product # PA5-34791) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDK7 Polyclonal Antibody immunoprecipitates CDK7 protein in IP experiments. IP samples: 293T whole cell extract. A. 40 µg 293T whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of CDK7 protein by 4 µg CDK7 Polyclonal Antibody (Product # PA5-34791). 10 % SDS-PAGE. The immunoprecipitated CDK7 protein was detected by CDK7 Polyclonal Antibody (Product # PA5-34791) diluted at 1:500.
