Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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Validation data
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- Product number
- MA3-001 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK7 Monoclonal Antibody (31TF2-1F8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA3-001 has been used successfully in Western blotting to detect Cyclin dependent kinase 7 in human lysates.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 31TF2-1F8
- Vial size
- 50 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Response of Two Mytilids to a Heatwave: The Complex Interplay of Physiology, Behaviour and Ecological Interactions.
Sublethal stress: Impact of solar UV radiation on protein synthesis in the copepod Acartia tonsa.
Effects of neurotoxic insecticides on heat-shock proteins and cytokine transcription in Chinook salmon (Oncorhynchus tshawytscha).
The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae.
Effects of environmental stress on intertidal mussels and their sea star predators.
Pesticide and pathogen: heat shock protein expression and acetylcholinesterase inhibition in juvenile Chinook salmon in response to multiple stressors.
An inducible 70 kDa-class heat shock protein is constitutively expressed during early development and diapause in the annual killifish Austrofundulus limnaeus.
Comparisons of stress proteins and soluble carbohydrate in encysted embryos of Artemia franciscana and two species of Parartemia.
Turning up the heat: the effects of thermal acclimation on the kinetics of hsp70 gene expression in the eurythermal goby, Gillichthys mirabilis.
Temperature differentially affects adenosine triphosphatase activity in Hsc70 orthologs from Antarctic and New Zealand notothenioid fishes.
Desiccation tolerance in encysted embryos of the animal extremophile, artemia.
Mytilus trossulus hsp70 as a biomarker for arsenic exposure in the marine environment: laboratory and real-world results.
Disruption of the endoplasmic reticulum by cytotoxins in LLC-PK1 cells.
Sublethal toxicity of orchard stormwater runoff in Sacramento splittail (Pogonichthys macrolepidotus) larvae.
Magnitude and duration of thermal stress determine kinetics of hsp gene regulation in the goby Gillichthys mirabilis.
Habitat diversity and adaptation to environmental stress in encysted embryos of the crustacean Artemia.
Molecular and cellular biomarker responses to pesticide exposure in juvenile chinook salmon (Oncorhynchus tshawytscha).
The influence of salinity on the heat-shock protein response of Potamocorbula amurensis (Bivalvia).
Biomarker responses in Macoma nasuta (Bivalvia) exposed to sediments from northern San Francisco Bay.
Heat-shock protein 70 (Hsp70) as a biochemical stress indicator: an experimental field test in two congeneric intertidal gastropods (genus: Tegula).
Small heat shock protein p26 associates with nuclear lamins and HSP70 in nuclei and nuclear matrix fractions from stressed cells.
Effects of dietary exposure to the pyrethroid pesticide esfenvalerate on medaka (Oryzias latipes).
Heat shock cognate protein 70 is involved in rotavirus cell entry.
Heat shock cognate protein 70 is involved in rotavirus cell entry.
Expression of 70 kDa heat shock proteins in antarctic and New Zealand notothenioid fish.
Heat-shock protein expression is absent in the antarctic fish Trematomus bernacchii (family Nototheniidae).
Effects of the Bacterial Extract OM-85 on Phagocyte Functions and the Stress Response.
Olabarria C, Gestoso I, Lima FP, Vázquez E, Comeau LA, Gomes F, Seabra R, Babarro JM
PloS one 2016;11(10):e0164330
PloS one 2016;11(10):e0164330
Sublethal stress: Impact of solar UV radiation on protein synthesis in the copepod Acartia tonsa.
Tartarotti B, Torres JJ
Journal of experimental marine biology and ecology 2009 Jul 15;375(1-2):106-113
Journal of experimental marine biology and ecology 2009 Jul 15;375(1-2):106-113
Effects of neurotoxic insecticides on heat-shock proteins and cytokine transcription in Chinook salmon (Oncorhynchus tshawytscha).
Eder KJ, Leutenegger CM, Köhler HR, Werner I
Ecotoxicology and environmental safety 2009 Jan;72(1):182-190
Ecotoxicology and environmental safety 2009 Jan;72(1):182-190
The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae.
Zhang H, Hu B, Ji Y, Kato A, Song Y
Cellular & molecular biology letters 2008;13(4):621-31
Cellular & molecular biology letters 2008;13(4):621-31
Effects of environmental stress on intertidal mussels and their sea star predators.
Petes LE, Mouchka ME, Milston-Clements RH, Momoda TS, Menge BA
Oecologia 2008 Jun;156(3):671-80
Oecologia 2008 Jun;156(3):671-80
Pesticide and pathogen: heat shock protein expression and acetylcholinesterase inhibition in juvenile Chinook salmon in response to multiple stressors.
Eder KJ, Köhler HR, Werner I
Environmental toxicology and chemistry 2007 Jun;26(6):1233-42
Environmental toxicology and chemistry 2007 Jun;26(6):1233-42
An inducible 70 kDa-class heat shock protein is constitutively expressed during early development and diapause in the annual killifish Austrofundulus limnaeus.
Podrabsky JE, Somero GN
Cell stress & chaperones 2007 Autumn;12(3):199-204
Cell stress & chaperones 2007 Autumn;12(3):199-204
Comparisons of stress proteins and soluble carbohydrate in encysted embryos of Artemia franciscana and two species of Parartemia.
Clegg JS, Campagna V
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 2006 Oct;145(2):119-25
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 2006 Oct;145(2):119-25
Turning up the heat: the effects of thermal acclimation on the kinetics of hsp70 gene expression in the eurythermal goby, Gillichthys mirabilis.
Lund SG, Ruberté MR, Hofmann GE
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2006 Apr;143(4):435-46
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2006 Apr;143(4):435-46
Temperature differentially affects adenosine triphosphatase activity in Hsc70 orthologs from Antarctic and New Zealand notothenioid fishes.
Place SP, Hofmann GE
Cell stress & chaperones 2005 Summer;10(2):104-13
Cell stress & chaperones 2005 Summer;10(2):104-13
Desiccation tolerance in encysted embryos of the animal extremophile, artemia.
Clegg JS
Integrative and comparative biology 2005 Nov;45(5):715-24
Integrative and comparative biology 2005 Nov;45(5):715-24
Mytilus trossulus hsp70 as a biomarker for arsenic exposure in the marine environment: laboratory and real-world results.
La Porte PF
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 2005 Nov-Dec;10(6):417-28
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 2005 Nov-Dec;10(6):417-28
Disruption of the endoplasmic reticulum by cytotoxins in LLC-PK1 cells.
Ryan PM, Bedard K, Breining T, Cribb AE
Toxicology letters 2005 Nov 15;159(2):154-63
Toxicology letters 2005 Nov 15;159(2):154-63
Sublethal toxicity of orchard stormwater runoff in Sacramento splittail (Pogonichthys macrolepidotus) larvae.
Teh SJ, Deng D, Werner I, Teh F, Hung SS
Marine environmental research 2005 Apr;59(3):203-16
Marine environmental research 2005 Apr;59(3):203-16
Magnitude and duration of thermal stress determine kinetics of hsp gene regulation in the goby Gillichthys mirabilis.
Buckley BA, Hofmann GE
Physiological and biochemical zoology : PBZ 2004 Jul-Aug;77(4):570-81
Physiological and biochemical zoology : PBZ 2004 Jul-Aug;77(4):570-81
Habitat diversity and adaptation to environmental stress in encysted embryos of the crustacean Artemia.
Tanguay JA, Reyes RC, Clegg JS
Journal of biosciences 2004 Dec;29(4):489-501
Journal of biosciences 2004 Dec;29(4):489-501
Molecular and cellular biomarker responses to pesticide exposure in juvenile chinook salmon (Oncorhynchus tshawytscha).
Eder KJ, Leutenegger CM, Wilson BW, Werner I
Marine environmental research 2004 Aug-Dec;58(2-5):809-13
Marine environmental research 2004 Aug-Dec;58(2-5):809-13
The influence of salinity on the heat-shock protein response of Potamocorbula amurensis (Bivalvia).
Werner I
Marine environmental research 2004 Aug-Dec;58(2-5):803-7
Marine environmental research 2004 Aug-Dec;58(2-5):803-7
Biomarker responses in Macoma nasuta (Bivalvia) exposed to sediments from northern San Francisco Bay.
Werner I, Teh SJ, Datta S, Lu X, Young TM
Marine environmental research 2004 Aug-Dec;58(2-5):299-304
Marine environmental research 2004 Aug-Dec;58(2-5):299-304
Heat-shock protein 70 (Hsp70) as a biochemical stress indicator: an experimental field test in two congeneric intertidal gastropods (genus: Tegula).
Tomanek L, Sanford E
The Biological bulletin 2003 Dec;205(3):276-84
The Biological bulletin 2003 Dec;205(3):276-84
Small heat shock protein p26 associates with nuclear lamins and HSP70 in nuclei and nuclear matrix fractions from stressed cells.
Willsie JK, Clegg JS
Journal of cellular biochemistry 2002;84(3):601-14
Journal of cellular biochemistry 2002;84(3):601-14
Effects of dietary exposure to the pyrethroid pesticide esfenvalerate on medaka (Oryzias latipes).
Werner I, Geist J, Okihiro M, Rosenkranz P, Hinton DE
Marine environmental research 2002 Sep-Dec;54(3-5):609-14
Marine environmental research 2002 Sep-Dec;54(3-5):609-14
Heat shock cognate protein 70 is involved in rotavirus cell entry.
Guerrero CA, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, Romero P, Méndez E, López S, Arias CF
Journal of virology 2002 Apr;76(8):4096-102
Journal of virology 2002 Apr;76(8):4096-102
Heat shock cognate protein 70 is involved in rotavirus cell entry.
Guerrero CA, Bouyssounade D, Zárate S, Isa P, López T, Espinosa R, Romero P, Méndez E, López S, Arias CF
Journal of virology 2002 Apr;76(8):4096-102
Journal of virology 2002 Apr;76(8):4096-102
Expression of 70 kDa heat shock proteins in antarctic and New Zealand notothenioid fish.
Carpenter CM, Hofmann GE
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2000 Feb;125(2):229-38
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2000 Feb;125(2):229-38
Heat-shock protein expression is absent in the antarctic fish Trematomus bernacchii (family Nototheniidae).
Hofmann GE, Buckley BA, Airaksinen S, Keen JE, Somero GN
The Journal of experimental biology 2000 Aug;203(Pt 15):2331-9
The Journal of experimental biology 2000 Aug;203(Pt 15):2331-9
Effects of the Bacterial Extract OM-85 on Phagocyte Functions and the Stress Response.
Baladi S, Kantengwa S, Donati YR, Polla BS
Mediators of inflammation 1994;3(2):143-8
Mediators of inflammation 1994;3(2):143-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK7 was performed by loading 50 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with Starting Block T20 (Product # 37543) for 1 hour at room temperature. CDK7 was detected at ~38 kD using a CDK7 monoclonal antibody (Product # MA3-001) at a dilution of 1:1000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK7 was performed by loading 50 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a PVDF membrane using the G2 Blotter (Product # 62288), and blocked with Starting Block T20 (Product # 37543) for 1 hour at room temperature. CDK7 was detected at ~38 kD using a CDK7 monoclonal antibody (Product # MA3-001) at a dilution of 1:1000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG Secondary Antibody, HRP conjugate (Product # 31430) at a dilution of 1:20,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product # 34078) and the myECL Imager (Product # 62236).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CDK7 was achieved by transfecting A-431 with CDK7 specific siRNAs (Silencer® select Product # s2830). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK7.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001) and a 39 kDa band corresponding to CDK7 was observed across cell lines and tissue extracts tested along with a 25kDa band which corresponds to free IgG. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), HeLa (Lane 4), Jurkat (Lane 5), HCT116 (Lane 6), K-562 (Lane 7), tissue extracts of Mouse Heart (Lane 8), Mouse Testis (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution), 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL