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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- A700-006 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK7 Recombinant Rabbit Monoclonal Antibody (BL-80-3D4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Antibody clone number
- BL-80-3D4
- Concentration
- 40 µg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK7 by WB of HeLa, Jurkat, MCF7, A549, SK-MEL28 (SK), and HCT116 [HCT] lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK7 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: ReliaBLOT® reagents (WB120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK7 by WB of HeLa, Jurkat, MCF7, A549, SK-MEL28 (SK), and HCT116 [HCT] lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK7 in FFPE breast carcinoma cell line MCF-7 by ICC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK7 in FFPE human ovarian carcinoma by IHC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CDK7 by flow cytometry. Sample: MOLT4 cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 µL of rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (Product # A700-006; lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (Product # A120-201D5).
