A700-006

antibody from Invitrogen Antibodies

Targeting: CDK7 CAK, CAK1, CDKN7, MO15, STK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-006

Provider

Invitrogen Antibodies

Product name

CDK7 Recombinant Rabbit Monoclonal Antibody (BL-80-5D4)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BL-80-5D4

Vial size

100 µL

Concentration

40 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK7 by WB of HeLa, Jurkat, MCF7, A549, SK-MEL28 (SK), and HCT116 [HCT] lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK7 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK7 by WB of HeLa, Jurkat, MCF7, A549, SK-MEL28 (SK), and HCT116 [HCT] lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK7 in FFPE breast carcinoma cell line MCF-7 by ICC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK7 in FFPE human ovarian carcinoma by IHC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (A700-006). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CDK7 by flow cytometry. Sample: MOLT4 cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 µL of rabbit anti-CDK7 recombinant monoclonal antibody [BL-80-5D4] (Product # A700-006; lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG (Product # A120-201D5).