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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [8]
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- Product number
- NBP1-86666 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86666, RRID:AB_11031324
- Product name
- Rabbit Polyclonal Cdk7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Cdk7 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cdk7 Antibody [NBP1-86666] - Analysis in human cell lines Caco-2 and MCF-7 using anti-CDK7 antibody. Corresponding CDK7 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cdk7 Antibody [NBP1-86666] - Analysis in control (vector only transfected HEK293T lysate) and CDK7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human cerebral cortex shows strong nuclear and moderate cytoplasmic positivity in neuronal cells. Glial cells showed weaker staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human gallbladder shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human liver shows very weak positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining in human testis and liver tissues using NBP1-86666 antibody. Corresponding CDK7 RNA-seq data are presented for the same tissues.
