Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F54805 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-CDK7 Antibody (pT170)
- Antibody type
- Polyclonal
- Description
- This highly specific Phospho-CDK7 antibody is suitable for use in Immunohistochemistry/Dot blot/Western blot applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the phospho-CDK7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of lysate from mouse NIH 3T3 cells treated or non-treated with lambda protein phosphatase with phospho-CDK7 antibody. Expected molecular weight ~39 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of lysate from human HeLa and MCF7 cells treated or non-treated with lambda protein phosphatase with phospho-CDK7 antibody. Expected molecular weight ~39 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast tissue with phospho-CDK7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of phospho-CDK7 antibody. 50 nanograms of phos-peptide or nonphos-peptide per dot were spotted.
