Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 653502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#653502, RRID:AB_2561763
- Product name
- Purified anti-Nur77
- Antibody type
- Monoclonal
- Antigen
- Partial human Nur77 recombinant protein (1-266 aa)
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1E10A15
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates from untreated Jurkat cells (Lane 1) or Jurkat cells treated with PMA and calcium ionophore (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Nur77 (clone 1E10A15) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated