653504

antibody from BioLegend

Targeting: NR4A1 GFRP1, HMR, N10, NAK-1, NGFIB, NUR77, TR3

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

653504

Provider

BioLegend

Proper citation

BioLegend Cat#653504, RRID:AB_2563321

Product name

Alexa Fluor® 594 anti-Nur77

Antibody type

Monoclonal

Antigen

Partial human Nur77 recombinant protein (1-266 aa)

Description

Nur77, a transcription factor belonging to a superfamily of orphan nuclear receptors, has been shown to activate a subset of genes containing NGFI-B response element (NBRE). Nur77 expression is upregulated in response to stimuli of numerous growth factors, mitogens, and inflammatory substances, indicating that Nur77 has regulatory roles in cell growth and inflammation. Nur77 has also been shown to participate in programmed cell death through mitochondrial targeting and interaction with Bcl-2. In the endocrine system, Nur77 has been reported to participate in regulating production of steroidogenic enzymes. Nur77 and NOR-1 double knockout mice develop acute myeloid leukemia, suggesting that Nur77 plays a role in regulating differentiation of myeloid progenitor cells.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

1E10A15

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-Nur77 (clone 1E10A15) Alexa Fluor® 594 (red) in blocking buffer, overnight at 4°C, and followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.

Conjugate

Yellow dye