12235-1-AP

antibody from Proteintech Group

Targeting: NR4A1 GFRP1, HMR, N10, NAK-1, NGFIB, NUR77, TR3

Provider product page for 12235-1-AP

Western blot

ELISA

Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [53]
Comments [0]
Validations
Western blot [1]

Submit

Product number: 12235-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12235-1-AP, RRID:AB_10644125
Product name: NR4A1 antibody
Antibody type: Polyclonal
Description: KD/KO validated NR4A1 antibody (Cat. #12235-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NR4A1 protein structure - 12235-1-AP shown in red.

Submitted references Nur77 attenuates Paneth cell necroptosis-induced intestinal inflammation through regulating endoplasmic reticulum homeostasis in sepsis.

Cui C, Huo Q, Ran S, Wang W, Wei H, Peng J
Journal of advanced research 2026 May;83:269-285

KMO downregulation promotes hepatocellular carcinoma growth via 3‑HAA‑mediated mitochondrial mass and function imbalances

Liu M, Zhang X, Hu Y, Niu L, Guo L, Zhao G, Lu F, Jia F, Li L, Wu H, Zhu J
International journal of oncology 2026 Mar;68(3)

Integrated multi-omics analysis identifies and validates endoplasmic reticulum stress and mitophagy-related biomarkers in MASLD.

Chen Q, Liu L, Feng J, Zhang C, Zhang Z
Scientific reports 2026 Mar 8;16(1)

Design, synthesis and evaluation of Nur77 modulators for lysosome dysfunction-driven therapy of gastric cancer.

Huang J, Zhang Z, Chen K, Zhang Y, Qin J, Hu H
European journal of medicinal chemistry 2026 Jul 5;311:118823

Stress-Induced Activation of Prolactin-NR4A1-Midkine Axis Exacerbates Skin Inflammation.

Li Z, Quan H, Liu W, Chen J, Chu M, Tang X, Xue K, Liu X, Ma J, Bai Y, Dong R, Li B, Hao J, Guo W, Li Q, Dang E, Gudjonsson JE, Wang G, Shao S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(5):e09679

Nr4a1 β-cell specific deletion impairs glucose tolerance in female mice.

Herring JA, Wynn AG, Aitken TJ, Walker ND, Whalen KE, Tessem JS
American journal of physiology. Endocrinology and metabolism 2026 Feb 1;330(2):E179-E194

METTL16 improves cigarette smoking-induced COPD emphysema through inhibiting autophagy by mediating pri-miR-511 m6A modification.

Cai X, Huang B, Gao F, Hu J, Yang F, Tang Y, Yu J, Tan X, Wang C, Qin H
International immunopharmacology 2026 Apr 1;174:116397

Lentinan inhibits melanoma development by regulating the AKT/Nur77/Bcl-2 signaling axis.

Lai X, Chen Y, Huang R, Zhu F, Huang L, Chen N, Li G, Hou W, Pan Y, Alitongbieke G
Journal of Cancer 2025;16(2):543-557

Protecting the brain from stroke: Huoxue Rongluo formula (HXRLF) targets ferroptosis for neuroprotection.

Ma Q, Luo L, Liao Y, Yang R, Ouyang X, Zhang Y, Yan S, Gong C, Liu L, Zhou D
Journal of ethnopharmacology 2025 Sep 25;353(Pt A):120329

EOAI3402143 inhibits lung adenocarcinoma progression through the NF-κB/NR4A1 pathway.

Xu J, Liu W, Chen L, Zhang J, Zhang D, Huang H, Zhang X, Huang X, Zhang G
Acta biochimica et biophysica Sinica 2025 Oct 9;58(4):833-44

Development of a prognostic model based on seven mitochondrial autophagy- and ferroptosis-related genes in lung adenocarcinoma.

Zhao YN, Li HY, Liu D, Feng K, Wang H, Liu TW, Li Y, Wang JY, Hou BF
BMC medical genomics 2025 Oct 1;18(1):145

Exogenous α-Synuclein Induces Oxidative Damage to Dopaminergic Neurons Through p-NMDAR2B/Nur77.

Lai X, Wu J, Kou X, Zhang Y, Shen M, Yu M, Zhai Y, Yan J
Molecular neurobiology 2025 May;62(5):5664-5678

Nuclear receptor 4A1 facilitates complete Freund's adjuvant-induced inflammatory pain in rats by promoting ferroptosis in spinal glial cells.

Deng Y, Xuan R, Qiu Z, Xiang P, Guo Y, Xu L, Zhang X, Mai H, Li X
Brain, behavior, and immunity 2025 Mar;125:92-109

Polystyrene microplastics induce hepatic lipid metabolism and energy disorder by upregulating the NR4A1-AMPK signaling pathway.

Chiu HW, Chu CW, Huang CC, Chia ZC, Wang YL, Lee YH
Environmental pollution (Barking, Essex : 1987) 2025 Mar 15;369:125850

NR4A1 Mediates Bronchopulmonary Dysplasia-Like Lung Injury Induced by Intrauterine Inflammation in Mouse Offspring.

Ding X, Li R, Yao D, Lei Z, Li W, Shen Q, Chen Z, Ni M, Li B, Liu X, Zhao J, Zhang Q, Liu Z
International journal of molecular sciences 2025 Jul 18;26(14)

HPGD: An Intermediate Player in Microglial Polarization and Multiple Sclerosis Regulated by Nr4a1.

Sun M, Liu Y, Wang X, Wang L
Molecular neurobiology 2025 Jan;62(1):271-287

Dual nuclear receptor 4A1 (NR4A1/NR4A2) ligands inhibit glioblastoma growth and target TWIST1.

Upadhyay S, Lee M, Zhang L, Oany AR, Mikheeva SA, Mikheev AM, Rostomily RC, Safe S
Molecular pharmacology 2025 Feb;107(2):100009

Laboratory Investigation of Lipid Metabolic Reprogramming in Pulpitis: The Linolenic Acid-FASN-NR4A1 Axis in Modulating Dental Pulp Inflammation.

Zhou M, Sui X, Li C, Zhou Q, Wei X, Xu H
International endodontic journal 2025 Dec;58(12):1913-1925

NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasis.

Jiang C, Zhu Y, Zhang J, Chen H, Li W, Xie R, Kong L, Chen L, Chen X, Huang H, Xu S
Experimental & molecular medicine 2025 Apr;57(4):804-819

Characterization of the Immune Microenvironment and Identification of Biomarkers in Chronic Rhinosinusitis with Nasal Polyps Using Single-Cell RNA Sequencing and Transcriptome Analysis.

Wang Y, Song X, Jin M, Lu J
Journal of inflammation research 2024;17:253-277

Nuclear receptor 4A1 ameliorates renal fibrosis by inhibiting vascular endothelial growth factor A induced angiogenesis in UUO rats.

Wang H, Fang F, Zhang M, Xu C, Liu J, Gao L, Zhao C, Wang Z, Zhong Y, Wang X
Biochimica et biophysica acta. Molecular cell research 2024 Oct;1871(7):119813

Characterization of PANoptosis-related genes with immunoregulatory features in osteoarthritis.

Lan Z, Yang Y, Sun R, Lin X, Yan J, Chen X, Tian K, Wu G, Saad M, Wu Z, Xue D, Jin Q
International immunopharmacology 2024 Oct 25;140:112889

Loss of glucose-stimulated β-cell Nr4a1 expression impairs insulin secretion and glucose homeostasis.

Herring JA, Crabtree JE, Hill JT, Tessem JS
American journal of physiology. Cell physiology 2024 Oct 1;327(4):C1111-C1124

Integrated bioinformatics analysis and in vivo validation of potential immune-related genes linked to diabetic nephropathy.

Deng J, Wu P
Heliyon 2024 Nov 15;10(21):e40151

Nur77 inhibition of β-catenin expression mediates Hepatoblastoma progression and enhances cisplatin's therapeutic effect.

Zhou J, Liu X, Yin H, Zhou Y, Xu Z, Zhou K, Li T, Fang Y, Shen Q
Gene 2024 May 25;908:148292

Structure-based design and synthesis of anti-fibrotic compounds derived from para-positioned 3,4,5-trisubstituted benzene.

Hong W, Xiao T, Lin G, Liu C, Li H, Li Y, Hu H, Wu S, Wang S, Liang Z, Lin T, Liu J, Chen X
Bioorganic chemistry 2024 Mar;144:107113

Spinosin inhibits activated hepatic stellate cell to attenuate liver fibrosis by targeting Nur77/ASK1/p38 MAPK signaling pathway.

Lin G, Li W, Hong W, Zhu D, Hu H, Fu J, Gao Y, Chen S, Chai D, Zeng JZ
European journal of pharmacology 2024 Mar 5;966:176270

A Graphene Composite Film Based Wearable Far-Infrared Therapy Apparatus (GRAFT) for Effective Prevention of Postoperative Peritoneal Adhesion.

Lu X, Xu L, Song Y, Yu X, Li Q, Liu F, Li X, Xi J, Wang S, Wang L, Wang Z
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jun;11(22):e2309330

In Silico Discovery of Stapled Peptide Inhibitor Targeting the Nur77-PPARγ Interaction and Its Anti-Breast-Cancer Efficacy.

Bian H, Liang X, Lu D, Lin J, Lu X, Jin J, Zhang L, Wu Y, Chen H, Zhang W, Luan X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jul;11(26):e2308435

Tasquinimod enhances the sensitivity of ovarian cancer cells to cisplatin by regulating the Nur77-Bcl-2 apoptotic pathway.

Lin Y, Liu YQ, Zhu KA, Hu MQ, Li Z, Min XJ
Advances in clinical and experimental medicine : official organ Wroclaw Medical University 2024 Feb;33(2):151-161

Identification of (E)-1-((1H-indol-3-yl)methylene)-4-substitute-thiosemicarbazones as potential anti-hepatic fibrosis agents.

Lin G, Zhong Y, Hu S, He F, Zhang Z, Li W, Hu H, Zeng JZ
Bioorganic chemistry 2024 Feb;143:107022

CEBPA Overexpression Enhances β-Cell Proliferation and Survival.

Ellsworth PN, Herring JA, Leifer AH, Ray JD, Elison WS, Poulson PD, Crabtree JE, Van Ry PM, Tessem JS
Biology 2024 Feb 9;13(2)

SPDEF ameliorates UUO-induced renal fibrosis by transcriptional activation of NR4A1.

Wang H, Wei Z, Xu C, Fang F, Wang Z, Zhong Y, Wang X
Molecular medicine (Cambridge, Mass.) 2024 Dec 30;30(1):282

A long-acting FGF21 attenuates metabolic dysfunction-associated steatohepatitis-related fibrosis by modulating NR4A1-mediated Ly6C phenotypic switch in macrophages.

Ji Y, Duan Y, Li Y, Lu Q, Liu D, Yang Y, Chang R, Tian J, Yao W, Yin J, Gao X
British journal of pharmacology 2024 Aug;181(16):2923-2946

Nur77 Mediates Anaphylaxis by Regulating miR-21a.

Jo H, Jeoung J, Shim K, Jeoung D
Current issues in molecular biology 2024 Apr 6;46(4):3175-3192

Design, synthesis, and biological evaluation of carbonyl-hydrazine-1-carboxamide derivatives as anti-hepatic fibrosis agents targeting Nur77.

Hu H, Lin G, He F, Liu J, Jia R, Li K, Hong W, Fang M, Zeng JZ
Bioorganic chemistry 2023 Nov;140:106795

β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals.

Zhu F, Zhang Q, Feng J, Zhang X, Li T, Liu S, Chen Y, Li X, Wu Q, Xue Y, Alitongbieke G, Pan Y
Immunity, inflammation and disease 2023 May;11(5):e876

Hederagenin suppresses glioma cell biological activities via Nur77 in vitro study.

Dai Y, Masra N, Zhou L, Yu C, Jin W, Ni H
Food science & nutrition 2023 Mar;11(3):1283-1296

Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver.

Sheng M, Weng Y, Cao Y, Zhang C, Lin Y, Yu W
Cell death discovery 2023 Mar 28;9(1):106

HK1 from hepatic stellate cell-derived extracellular vesicles promotes progression of hepatocellular carcinoma.

Chen QT, Zhang ZY, Huang QL, Chen HZ, Hong WB, Lin T, Zhao WX, Wang XM, Ju CY, Wu LZ, Huang YY, Hou PP, Wang WJ, Zhou D, Deng X, Wu Q
Nature metabolism 2022 Oct;4(10):1306-1321

The epilepsy-associated protein PCDH19 undergoes NMDA receptor-dependent proteolytic cleavage and regulates the expression of immediate-early genes.

Gerosa L, Mazzoleni S, Rusconi F, Longaretti A, Lewerissa E, Pelucchi S, Murru L, Giannelli SG, Broccoli V, Marcello E, Kasri NN, Battaglioli E, Passafaro M, Bassani S
Cell reports 2022 May 24;39(8):110857

hUCMSCs reduce theca interstitial cells apoptosis and restore ovarian function in premature ovarian insufficiency rats through regulating NR4A1-mediated mitochondrial mechanisms.

Luo Q, Tang Y, Jiang Z, Bao H, Fu Q, Zhang H
Reproductive biology and endocrinology : RB&E 2022 Aug 19;20(1):125

TIAM1-RAC1 promote small-cell lung cancer cell survival through antagonizing Nur77-induced BCL2 conformational change.

Payapilly A, Guilbert R, Descamps T, White G, Magee P, Zhou C, Kerr A, Simpson KL, Blackhall F, Dive C, Malliri A
Cell reports 2021 Nov 9;37(6):109979

A systematic dissection of human primary osteoblasts in vivo at single-cell resolution.

Gong Y, Yang J, Li X, Zhou C, Chen Y, Wang Z, Qiu X, Liu Y, Zhang H, Greenbaum J, Cheng L, Hu Y, Xie J, Yang X, Li Y, Bai Y, Wang YP, Chen Y, Tan LJ, Shen H, Xiao HM, Deng HW
Aging 2021 Aug 24;13(16):20629-20650

Simvastatin Improves Behavioral Disorders and Hippocampal Inflammatory Reaction by NMDA-Mediated Anti-inflammatory Function in MPTP-Treated Mice.

Yan J, Liu A, Fan H, Qiao L, Wu J, Shen M, Lai X, Huang J
Cellular and molecular neurobiology 2020 Oct;40(7):1155-1164

Ovarian tumor domain-containing ubiquitin aldehyde binding protein 1 inhibits inflammation by regulating Nur77 stability.

Pei HZ, Huang B, Chang HW, Baek SH
Cellular signalling 2019 Jul;59:85-95

Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice.

Kameda H, Yamamoto M, Tone Y, Tone M, Melmed S
Endocrinology 2019 Feb 1;160(2):276-291

The E3 ubiquitin ligase Trim13 regulates Nur77 stability via casein kinase 2α.

Huang B, Pei HZ, Chang HW, Baek SH
Scientific reports 2018 Sep 17;8(1):13895

Nur77 exacerbates PC12 cellular injury in vitro by aggravating mitochondrial impairment and endoplasmic reticulum stress.

Gao H, Chen Z, Fu Y, Yang X, Weng R, Wang R, Lu J, Pan M, Jin K, McElroy C, Tang B, Xia Y, Wang Q
Scientific reports 2016 Sep 29;6:34403

Contra-directional Coupling of Nur77 and Nurr1 in Neurodegeneration: A Novel Mechanism for Memantine-Induced Anti-inflammation and Anti-mitochondrial Impairment.

Wei X, Gao H, Zou J, Liu X, Chen D, Liao J, Xu Y, Ma L, Tang B, Zhang Z, Cai X, Jin K, Xia Y, Wang Q
Molecular neurobiology 2016 Nov;53(9):5876-5892

NR4A1 Knockdown Suppresses Seizure Activity by Regulating Surface Expression of NR2B.

Zhang Y, Chen G, Gao B, Li Y, Liang S, Wang X, Wang X, Zhu B
Scientific reports 2016 Nov 23;6:37713

Antitumor activity of SAHA, a novel histone deacetylase inhibitor, against murine B cell lymphoma A20 cells in vitro and in vivo.

Yang B, Yu D, Liu J, Yang K, Wu G, Liu H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2015 Jul;36(7):5051-61

The Orphan Nuclear Receptor NR4A1 Protects Pancreatic β-Cells from Endoplasmic Reticulum (ER) Stress-mediated Apoptosis.

Yu C, Cui S, Zong C, Gao W, Xu T, Gao P, Chen J, Qin D, Guan Q, Liu Y, Fu Y, Li X, Wang X
The Journal of biological chemistry 2015 Aug 21;290(34):20687-20699

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The NUR77 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NUR77. This antibody recognizes human,mouse,rat antigen. The NUR77 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 12235-1-AP.pdf

: Show more