MA5-32647
antibody from Invitrogen Antibodies
Targeting: NR4A1
GFRP1, HMR, N10, NAK-1, NGFIB, NUR77, TR3
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [5]
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Validation data
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- Product number
- MA5-32647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUR77 Recombinant Rabbit Monoclonal Antibody (JM59-11)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JM59-11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Cardiac Transcriptome Analysis Reveals Nr4a1 Mediated Glucose Metabolism Dysregulation in Response to High-Fat Diet.
Men L, Hui W, Guan X, Song T, Wang X, Zhang S, Chen X
Genes 2020 Jun 29;11(7)
Genes 2020 Jun 29;11(7)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NUR77 in rat brain cell lysate using a NUR77 Monoclonal antibody (Product # MA5-32647) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NUR77 in Hela cells using a NUR77 Monoclonal antibody (Product # MA5-32647) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NUR77 in HepG2 cells using a NUR77 Monoclonal antibody (Product # MA5-32647) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NUR77 in Hela cells using a NUR77 Monoclonal antibody (Product # MA5-32647) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of NUR77 in NIH-3T3 cells using a NUR77 Monoclonal antibody (Product # MA5-32647) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NUR77 of paraffin-embedded Human liver tissue using a NUR77 Monoclonal antibody (Product #MA5-32647). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NUR77 of paraffin-embedded Human colon cancer tissue using a NUR77 Monoclonal antibody (Product #MA5-32647). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NUR77 of paraffin-embedded Human breast tissue using a NUR77 Monoclonal antibody (Product #MA5-32647). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NUR77 of paraffin-embedded Mouse testis tissue using a NUR77 Monoclonal antibody (Product #MA5-32647). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NUR77 of paraffin-embedded Mouse ovarian tissue using a NUR77 Monoclonal antibody (Product #MA5-32647). Counter stained with hematoxylin.