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LearnPA5-27274
antibody from Invitrogen Antibodies
Targeting: NR4A1
GFRP1, HMR, N10, NAK-1, NGFIB, NUR77, TR3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-27274 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NR4A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HepG2 (treatment 1% Hypoxia for 24 hr), NUR77 tansfected 293T cell, mouse liver. Predicted reactivity: Mouse (92%), Rat (94%), Dog (96%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.23 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Dexmedetomidine alleviates pulmonary ischemia-reperfusion injury through modulating the miR-21-5p/Nr4a1 signaling pathway.
Dong W, Yang H, Cheng M, Zhang X, Yin J, Zeng Z, Huang G
Acta biochimica Polonica 2020 Dec 17;67(4):521-529
Acta biochimica Polonica 2020 Dec 17;67(4):521-529
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NR4A1 Polyclonal Antibody (Product # PA5-27274). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 7.5% SDS PAGE. NR4A1 Polyclonal Antibody (Product # PA5-27274) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NR4A1 expression in transfected 293T cell line by NR4A1 polyclonal antibody. A: Non-transfected lysate. Lane B: NR4A1 transfected lysate. 12% SDS PAGE. NR4A1 Polyclonal Antibody (Product # PA5-27274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NR4A1 was performed by separating 30 µg of untreated (–) and treated (+) HepG2 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NR4A1 Polyclonal Antibody (Product # PA5-27274) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NUR77 in paraformaldehyde-fixed HeLa cells using a NUR77 polyclonal antibody (Product # PA5-27274) at a 1:500 dilution.
