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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-13130 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-13130, RRID:AB_627245
- Product name
- Anti-CDK9
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Suberoylanilide hydroxamic acid reactivates HIV from latently infected cells.
Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy.
Human cytomegalovirus infection induces specific hyperphosphorylation of the carboxyl-terminal domain of the large subunit of RNA polymerase II that is associated with changes in the abundance, activity, and localization of cdk9 and cdk7.
A model of repression: CTD analogs and PIE-1 inhibit transcriptional elongation by P-TEFb.
Contreras X, Schweneker M, Chen CS, McCune JM, Deeks SG, Martin J, Peterlin BM
The Journal of biological chemistry 2009 Mar 13;284(11):6782-9
The Journal of biological chemistry 2009 Mar 13;284(11):6782-9
Pivotal role of cardiac lineage protein-1 (CLP-1) in transcriptional elongation factor P-TEFb complex formation in cardiac hypertrophy.
Espinoza-Derout J, Wagner M, Shahmiri K, Mascareno E, Chaqour B, Siddiqui MA
Cardiovascular research 2007 Jul 1;75(1):129-38
Cardiovascular research 2007 Jul 1;75(1):129-38
Human cytomegalovirus infection induces specific hyperphosphorylation of the carboxyl-terminal domain of the large subunit of RNA polymerase II that is associated with changes in the abundance, activity, and localization of cdk9 and cdk7.
Tamrakar S, Kapasi AJ, Spector DH
Journal of virology 2005 Dec;79(24):15477-93
Journal of virology 2005 Dec;79(24):15477-93
A model of repression: CTD analogs and PIE-1 inhibit transcriptional elongation by P-TEFb.
Zhang F, Barboric M, Blackwell TK, Peterlin BM
Genes & development 2003 Mar 15;17(6):748-58
Genes & development 2003 Mar 15;17(6):748-58
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 43
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
