Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA1-84581 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CDK9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-84581 does not cross-react with other cyclin dependent kinases. Rabbit anti Human Cdk9 antibody recognizes a approximately 43 kDa member of the cyclin-dependent protein kinase (CDK) family, CDK9, previously named PITALRE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- 4°C or -20°C if preferred
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of CDK9 was performed by loading 50 µg of J-lat full (10.6) whole cell lysate in reducing sample buffer onto a 4-15% tris-glycine gel. Proteins were transferred to PVDF and then blocked in blocking buffer (TBST+5% non-fat milk) for one hour at room temperature. CDK9 was detected using an anti-CDK9 polyclonal antibody (Product # PA1-84581) at a dilution of 1:1,000 overnight at 4°C on a rocking platform. Goat anti Rabbit HRP-conjugated secondary antibody was diluted at 1:2000 in 1xTBST buffer with 5% non fat milk and incubated for 1 hour at room temperature. Data courtesy of Antibody Data Exchange Program.