PA1-84581

antibody from Invitrogen Antibodies

Targeting: CDK9 C-2k, CDC2L4, PITALRE, TAK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-84581

Provider

Invitrogen Antibodies

Product name

Anti-CDK9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-84581 does not cross-react with other cyclin dependent kinases. Rabbit anti Human Cdk9 antibody recognizes a approximately 43 kDa member of the cyclin-dependent protein kinase (CDK) family, CDK9, previously named PITALRE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

4°C or -20°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDK9 was performed by loading 50 µg of J-lat full (10.6) whole cell lysate in reducing sample buffer onto a 4-15% tris-glycine gel. Proteins were transferred to PVDF and then blocked in blocking buffer (TBST+5% non-fat milk) for one hour at room temperature. CDK9 was detected using an anti-CDK9 polyclonal antibody (Product # PA1-84581) at a dilution of 1:1,000 overnight at 4°C on a rocking platform. Goat anti Rabbit HRP-conjugated secondary antibody was diluted at 1:2000 in 1xTBST buffer with 5% non fat milk and incubated for 1 hour at room temperature. Data courtesy of Antibody Data Exchange Program.