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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319416 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal CDK9 phospho T29 antibody (Phospho-specific)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal CDK9 phospho T29 antibody (Phospho-specific)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CDK9
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.3mg/ml
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.
- Validation comment
- WB
