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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10000 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10000, RRID:AB_1676119
- Product name
- CDK9 (phospho T29) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDK9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Tax interacts with P-TEFb in a novel manner to stimulate human T-lymphotropic virus type 1 transcription.
The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription.
Differential localization and expression of the Cdk9 42k and 55k isoforms.
Zhou M, Lu H, Park H, Wilson-Chiru J, Linton R, Brady JN
Journal of virology 2006 May;80(10):4781-91
Journal of virology 2006 May;80(10):4781-91
The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription.
Jang MK, Mochizuki K, Zhou M, Jeong HS, Brady JN, Ozato K
Molecular cell 2005 Aug 19;19(4):523-34
Molecular cell 2005 Aug 19;19(4):523-34
Differential localization and expression of the Cdk9 42k and 55k isoforms.
Liu H, Herrmann CH
Journal of cellular physiology 2005 Apr;203(1):251-60
Journal of cellular physiology 2005 Apr;203(1):251-60
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Supportive validation
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- Experimental details
- Western blot using CDK9 (phospho T29) polyclonal antibody (Cat # PAB10000) shows detection of phospho-CDK9.100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29.The primary antibody was used at a 1:1000 dilution.Personal Communication, J. Brady, NCI, Bethesda, MD.
