PA5-85238

antibody from Invitrogen Antibodies

Targeting: CDK9 C-2k, CDC2L4, PITALRE, TAK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85238

Provider

Invitrogen Antibodies

Product name

CDK9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDK9 in A) NIH-3T3 cells using CDK9 polyclonal antibody (Product # PA5-85238) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CDK9 in A) HL-60 cells using CDK9 polyclonal antibody (Product # PA5-85238) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CDK9 Polyclonal Antibody (Product # PA5-85238). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 10% SDS PAGE. CDK9 Polyclonal Antibody (Product # PA5-85238) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CDK9 Polyclonal Antibody (Product # PA5-85238). Sample (30 µg of whole cell lysate). Lane A: HL-60. 10% SDS PAGE. CDK9 Polyclonal Antibody (Product # PA5-85238) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CDK9 Polyclonal Antibody detects CDK9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK9 protein stained by CDK9 Polyclonal Antibody (Product # PA5-85238) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CDK9 Polyclonal Antibody detects CDK9 protein at cytoplasm and nucleus in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. CDK9 Polyclonal Antibody (Product # PA5-85238) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CDK9 Polyclonal Antibody detects CDK9 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CDK9 Polyclonal Antibody (Product # PA5-85238) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.