MA5-42642

antibody from Invitrogen Antibodies

Targeting: CDK9 C-2k, CDC2L4, PITALRE, TAK

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42642

Provider

Invitrogen Antibodies

Product name

CDK9 Recombinant Rabbit Monoclonal Antibody (1C4E3)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive test controls include: HeLa, 293T, MCF7, Mouse brain, Rat testis, Rat brain. The target is usually found in the following locations: Cytoplasm, Nucleus, PML body. Immunogen sequence: RKVKDRLKAY VRDPYALDLI DKLLVLDPAQ RIDSDDALNH DFFWSDPMPS DLKGMLSTHL TSMFEYLAPP RRKGSQITQQ STNQSRNPAT TNQTEFERVF

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

1C4E3

Vial size

100 µL

Concentration

0.23 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal imaging (Immunocytochemistry) of CDK9 in U-2 OS cells. Samples were incubated with CDK9 Monoclonal antibody (Product # MA5-42642) using a dilution of 1:50 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CDK9 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg CDK9 Monoclonal antibody (Product # MA5-42642). Western blot was performed from the immunoprecipitate using CDK9 Monoclonal antibody (Product # MA5-42642) at a dilution of 1:1,000.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of CDK9 in extracts of DLD-1 cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642)The ChIP sequencing results indicate the enrichment pattern of CDK9 in selected genomic region and representative gene loci (e.g. RAC1, GAPDH and ACTB), as shown in figure.