MA5-42642
antibody from Invitrogen Antibodies
Targeting: CDK9
C-2k, CDC2L4, PITALRE, TAK
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- MA5-42642 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK9 Recombinant Rabbit Monoclonal Antibody (1C4E3)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: HeLa, 293T, MCF7, Mouse brain, Rat testis, Rat brain. The target is usually found in the following locations: Cytoplasm, Nucleus, PML body. Immunogen sequence: RKVKDRLKAY VRDPYALDLI DKLLVLDPAQ RIDSDDALNH DFFWSDPMPS DLKGMLSTHL TSMFEYLAPP RRKGSQITQQ STNQSRNPAT TNQTEFERVF
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 1C4E3
- Vial size
- 100 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDK9 in various cell lines using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CDK9 in HeLa cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CDK9 in human esophageal using a Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CDK9 in human esophageal using a Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CDK9 in human esophageal using a Recombinant Monoclonal antibody (Product # MA5-42642) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of CDK9 in extracts of DLD-1 cells using a CDK9 Recombinant Monoclonal antibody (Product # MA5-42642)The ChIP sequencing results indicate the enrichment pattern of CDK9 in selected genomic region and representative gene loci (e.g. RAC1, GAPDH and ACTB), as shown in figure.