MA5-15849

C-2k, CDC2L4, PITALRE, TAK

Antibody data

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Validation data

Product number: MA5-15849
Provider: Invitrogen Antibodies
Product name: Anti-CDK9 Monoclonal Antibody (1B5A7)
Provider product page: Invitrogen Antibodies - MA5-15849
Antibody type: Monoclonal
Antigen: Recombinant protein fragment
Description: MA5-15849 targets CDK9 in IHC and WB applications and shows reactivity with Human samples. The MA5-15849 immunogen is purified recombinant fragment of human CDK9 expressed in E. Coli. . MA5-15849 detects CDK9 which has a predicted molecular weight of approximately 43kDa.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 1B5A7
Vial size: 100 µL
Concentration: Lot Dependent
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CDK9 using CDK9 monoclonal antibody (Product # MA5-15849) in HEK293 (1) and CDK9 (AA: 178-369) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of CDK9 was achieved by transfecting HeLa cells with CDK9 specific siRNAs (Silencer® select Product # s2834, s2835). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with CDK9 Monoclonal Antibody (Product # MA5-15849, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK9.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), Raji (Lane 3), DU 145 (Lane 4) and Hep G2 (Lane 5). The blot was probed with Anti-CDK9 Monoclonal Antibody (Product # MA5-15849, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 43 kDa band corresponding to CDK9 was observed across the cell lines tested.