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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- MA5-15849 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK9 Monoclonal Antibody (1B5A7)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15849 targets CDK9 in IHC and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 1B5A7
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK9 using CDK9 monoclonal antibody (Product # MA5-15849) in HEK293 (1) and CDK9 (AA: 178-369) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), Raji (Lane 3), DU 145 (Lane 4) and Hep G2 (Lane 5). The blot was probed with Anti-CDK9 Monoclonal Antibody (Product # MA5-15849, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 43 kDa band corresponding to CDK9 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK9 using CDK9 monoclonal antibody (Product # MA5-15849) in HEK293 (1) and CDK9 (AA: 178-369) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of CDK9 was achieved by transfecting HeLa cells with CDK9 specific siRNAs (Silencer® select Product # s2834, s2835). Western blot analysis (Fig. a) was performed using whole cell extracts from the CDK9 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with CDK9 Monoclonal Antibody (Product # MA5-15849, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to CDK9.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues (left) and placenta tissues (right) using CDK9 monoclonal antibody (Product # MA5-15849) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using CDK9 monoclonal antibody (Product # MA5-15849) followed with DAB staining.
