R1184

antibody from Acris Antibodies GmbH

Targeting: CDK9 C-2k, CDC2L4, PITALRE, TAK

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

R1184

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1184, RRID:AB_977046

Product name

anti CDK9

Antibody type

Polyclonal

Antigen

Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the Human gene cdk9 (PITALRE).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

0.1 ml

Concentration

75 mg/ml (by Refractometry)

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunocytochemical staining of Mouse tissue using CDK9 antibody (PITALRE) antiserum. The staining shows the location of mcdk9/PITALRE protein in developing Mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing Mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Other detection systems should yield similar results. Sections from each specimen were cut at 5-7 μm, mounted on glass and dried overnight at 37°C. All sections then were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20°C for 1 h with Rabbit anti-CDK9 (1/500) dilution, washed, and then reacted with diluted Goat anti-Rabbit biotinylated antibody for 30 min. All the slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20°C. Diaminobenzidine was used as the final chromogen and hematoxylin was used as the nuclear counterstain. Negative controls for each tissue section were prepared by substituting the primary antiserum with preimmune serum.