AP09363PU-N

antibody from Acris Antibodies GmbH

Targeting: CDK9 C-2k, CDC2L4, PITALRE, TAK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AP09363PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09363PU-N, RRID:AB_2035346

Product name

anti CDK9 pThr29

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to residues surrounding Thr 29 in the Human CDK9 protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.3 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution.