Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AI10114 - Provider product page
- Provider
- Abgent
- Product name
- SIAH1 antibody - N-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Rabbit
- Host
- Rabbit
- Vial size
- 100 µg
- Storage
- "Add 100 ul of distilled water. Final anti-SIAH1 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles."
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "SIAH1 antibody - N-terminal region (AI10114) in Human HEK293T cells using Western BlotResearcher: Peter Brand & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University JenaApplication: Western blottingSpecies+tissue/cell type: Human embryonic kidney 293THow many ug⬠"!s of tissue/cell lysate run on the gel:1: 50 ug human HEK-293T cell lysatePrimary antibody dilution: 1:1000Secondary antibody: Anti-rabbit-IgGSecondary antibody dilution: 1:5000 - See more at: http://www.avivasysbio.com/anti-siah1-antibody-n-terminal-region-arp34163-t100.html#sthash.GIS7dILc.dpufSIAH1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells"
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "SIAH1 antibody - N-terminal region (AI10114) in Human Jurkat cells using Western BlotWB Suggested Anti-SIAH1 Antibody Titration: 1.25ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysate"
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "SIAH1 antibody (AI10114) in Human Heart cells using ImmunohistochemistryRabbit Anti-SIAH1 antibody Catalog Number: AI10114 Paraffin Embedded Tissue: Human Heart cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X"