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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-96137 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIAH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: Mouse kidney; Cellular Location: Cytoplasm, Nucleus Immunogen sequence: MTGKATPPSL YSWRGVLFTC LPAARTRKRK EMSRQTATAL PTGTSKCPPS QRVPALTGTT ASNNDLASLF ECPVCFDYVL PPILQCQSGH LVCSNCRPKL TCCPTCRGPL GSIRNLAMEK VANSVLFPCK YASSGCEITL PHTEKADHEE LCEFRPYSCP CPGASCKWQG SLDAVMPHLM HQHKSITTLQ GEDIVFLATD INLPGAVDWV MMQSCFGFHF MLVLEKQEKY DGHQQFFAIV QLIGTRKQAE NFAYRLELNG HRRRLTWEAT PRSIHEGIAT AIMNSDCLVF DTSIAQLFAE NGNLGINVTI SMC
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.03 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SIAH1 in extracts of mouse kidney using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SIAH1 was performed in U2OS cells using SIAH1 Polyclonal Antibody (Product # PA5-96137).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SIAH1 in paraffin-embedded rat brain using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SIAH1 in paraffin-embedded human colon carcinoma using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SIAH1 in paraffin-embedded mouse spleen using SIAH1 Polyclonal Antibody (Product # PA5-96137) at a dilution of 1:200.
