MA5-49361

antibody from Invitrogen Antibodies

Targeting: SIAH1 hSIAH1

Western blot

Antibody data

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Validation data

Product number: MA5-49361 - Provider product page
Provider: Invitrogen Antibodies
Product name: SIAH1 Recombinant Rabbit Monoclonal Antibody (PSH0-46)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Sequence Similarities: 100% Mouse/Rat. Tissue Specificity: Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas. Positive Control: Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, human brain tissue, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue, A549. Subcellular Location: Cytoplasm, Nucleus. Predicted band size: 31.1 kDa.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Antibody clone number: PSH0-46
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SIAH1 protein structure - MA5-49361 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SIAH1 was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:100 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2,000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SIAH1 using A549 cells. The cells were fixed 4% paraformaldehyde for 10 minutes, permeabilized with 0.05% Triton™ X-100 in PBS for 20 minutes, and blocked with 2% negative goat serum for 30 minutes at room temperature. The cells were labeled with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:100 dilutionin 2% negative goat serum overnight at 4°C and then with iFluor™ 488 Goat Anti-Rabbit IgG H&L secondary antibody (1:1,000 dilution) for 1 hour at room temperature (green). Nuclear were stained with DAPI (blue). Beta tubulin (red) was stained at 1:200 dilution overnight at +4°C. iFluor™ 594 Goat Anti-Mouse IgG H&L was used as the secondary antibody at 1:1,000 dilution. The images were captured at 200X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of SIAH1 on formalin-fixed paraffin-embedded human brain tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:1,000 dilution for 1 hour at room temperature. HRP conjugated compact polymer system and DAB chromogen were used as the detection system, followed by counterstaining with hematoxylin. The slide was mounted with DPX and the image was captured at 200X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of SIAH1 on formalin-fixed paraffin-embedded human testis tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:1,000 dilution for 1 hour at room temperature. HRP conjugated compact polymer system and DAB chromogen were used as the detection system, followed by counterstaining with hematoxylin. The slide was mounted with DPX and the image was captured at 200X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of SIAH1 on formalin-fixed paraffin-embedded mouse testis tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:1,000 dilution for 1 hour at room temperature. HRP conjugated compact polymer system and DAB chromogen were used as the detection system, followed by counterstaining with hematoxylin. The slide was mounted with DPX and the image was captured at 200X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of SIAH1 on formalin-fixed paraffin-embedded rat brain tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:1,000 dilution for 1 hour at room temperature. HRP conjugated compact polymer system and DAB chromogen were used as the detection system, followed by counterstaining with hematoxylin. The slide was mounted with DPX and the image was captured at 200X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of SIAH1 on formalin-fixed paraffin-embedded mouse brain tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:1,000 dilution for 1 hour at room temperature. HRP conjugated compact polymer system and DAB chromogen were used as the detection system, followed by counterstaining with hematoxylin. The slide was mounted with DPX and the image was captured at 200X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of SIAH1 on formalin-fixed paraffin-embedded rat testis tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with SIAH1 Rabbit Recombinant Monoclonal Antibody (PSH0-46) (Product # MA5-49361) at 1:1,000 dilution for 1 hour at room temperature. HRP conjugated compact polymer system and DAB chromogen were used as the detection system, followed by counterstaining with hematoxylin. The slide was mounted with DPX and the image was captured at 200X magnification.