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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Other assay [1]
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- Product number
- PA5-87308 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMG20A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.
Pan R, Yu D, Hu J, Yang X, Wang C, Zhang L, Xue P, Sun J, Zhang X, Cai W
Cell death discovery 2022 May 16;8(1):263
Cell death discovery 2022 May 16;8(1):263
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 6 HMG20A and SFMBT1 interact in vitro. A After 48 h transfection, the co-localization (yellow) of HMG20A (green) and SFMBT1 (red) were analyzed using fluorescent microscopy. Cell nuclei were stained by DAPI (blue). B Interaction of HMG20A and SFMBT1 in cells verified by co-immunoprecipitation. C , D Western blot analysis ( C ) and qRT-PCR ( D ) detect HMG20A protein and mRNA of downstream gene and protein after SFMBT1 knockdown. E , F Western blot and qRT-PCR analysis SFMBT1, HMG20A, EZH2 and FOXO1 protein ( E ) and mRNA levels ( F ) after SFMBT1 or HMG20A knockdown in SW620 and HCT-116 cells. * P < 0.05, ** P < 0.01, & P < 0.05, && P < 0.01.
