AM02057PU-N

antibody from Acris Antibodies GmbH

Targeting: IBSP BSP, BSP-II, SP-II

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

AM02057PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM02057PU-N, RRID:AB_1609496

Product name

anti Bone sialoprotein 2 (native)

Antibody type

Monoclonal

Antigen

Human Bone Sialoprotein (Bone extract).

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

ID1.2

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoblot analysis of AM02057PU-N/AM02057PU-S specificty. Native and recombinant Bone proteins were separated by SDS-PAGE and immunoblotted with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S at 1/4000 dilution. This antibody did not detect BSP purified from Porcine Bone, nor Porcine Osteopontin (OPN) or SPARC/Osteonectin (ON).Gogan G et al. (2004) Connect Tissue Res 45(1):60-71

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

ELISA standard curve showing measurement of Human BSP in a Sandwich Immunoassay using Cat.-No AM20240PU-N/AM20240PU-S as a Capture antibody and Cat.-No AM02057PU-N/AM02057PU-S as a Detection antibody. OD was determined at 450 nm. The shown standard curve is for demonstration purposes only and can be influenced by buffer, incubation conditions or dilution of the conjugate.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry image staining of BSP in Paraffin Section of Human Tissues. Sections were incubated with this BSP antibody Cat.-No AM02057PU-N/AM02057PU-S (1/2000) and detected using HRP conjugated secondary antibody and ABC Vectastain Elite Kit. DAB was used as the Chromogen. The sections were counterstained with Harris' Hematoxylin.Sections of B: Embryonic TibiaSections of E: Primary breast CarcinomaSections of G: Bone metastasis from the same patient were stained with this antibody.Inserted within B and E are Control Sections incubated with Mouse IgG. Scale bar = 20 µm.Gogan G et al. (2004) Connect Tissue Res 45(1):60-71