Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- R30987 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Ibsp Antibody / Bone sialoprotein 2
- Antibody type
- Polyclonal
- Description
- This highly specific Ibsp antibody is suitable for use in Western blot/Immunohistochemistry applications with mouse and rat samples.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Ibsp antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of BSP antibody and Lane 1: rat liver; 2: rat brain; 3: rat kidney tissue lysate. Observed size: 35~70KD depending on glycosylation level
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat cartilage and 2) mouse cartilage tissue lysate with Ibsp antibody. Expected molecular weight: 35~70 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: BSP antibody testing of rat liver tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse pancreas tissue with Ibsp antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat liver tissue with Ibsp antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.