PA5-79423

antibody from Invitrogen Antibodies

Targeting: IBSP BSP, BSP-II, SP-II

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79423

Provider

Invitrogen Antibodies

Product name

Bone SialoProtein Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Pulsed-electromagnetic-field induced osteoblast differentiation requires activation of genes downstream of adenosine receptors A2A and A3.

Kar NS, Ferguson D, Zhang N, Waldorff EI, Ryaby JT, DiDonato JA
PloS one 2021;16(2):e0247659

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bone SialoProtein in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: human HeLa whole cell lysate, Lane 4: human U2OS whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with Bone SialoProtein polyclonal antibody (Product # PA5-79423) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 Adora3 disruption affects PEMF mediated overexpression of BSP, IBSP and Osteocalcin. Wild-type (WT), Adora2 (Delta2), Adora3 (Delta3) and Double Knock-Out (DKO) MC3T3-E1 cells were treated with PEMF (4 hours daily) or differentiation medium (DM) for 12 days and Western Blots for fractionated whole-cell proteins were probed with each of the indicated antibodies.