PA5-79424

antibody from Invitrogen Antibodies

Targeting: IBSP BSP, BSP-II, SP-II

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79424

Provider

Invitrogen Antibodies

Product name

Bone SialoProtein Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - IHC: Human Osteosarcoma Tissue.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

500 μg/mL

Storage

-20°C

References

Submitted references

Impact of Marine-Based Biomaterials on the Immunoregulatory Properties of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Use of Fish Collagen in Bone Tissue Engineering.

Liu C, Sun J
ACS omega 2020 Nov 3;5(43):28360-28368

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Bone SialoProtein on paraffin-embedded human Osteosarcoma tissue. Sample was incubated with Bone SialoProtein polyclonal antibody (Product# PA5-79424).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Expression of TSG-6 in BMSCs and the effect of TSG-6 siRNA on the osteogenic differentiation of BMSCs. (A) mRNA expression levels of TSG-6 in BMSCs, BMSCs treated with transfection reagents only (BMSCs+TR), BMSCs transfected with scrambled siRNA (BMSCs+SS), and BMSCs transfected with TSG-6 siRNA (TSG-6-siRNA-BMSCs) were determined by real-time RT-PCR. Results are presented as the mean +- standard deviation of three independent experiments. * p < 0.05 compared with the BMSC group. (B) TSG-6 concentrations measured in the supernatants from BMSCs, BMSCs treated with HFC (BMSCs+HFC), TSG-6-siRNA-BMSCs treated with HFC (TSG-6-siRNA-BMSCs+HFC), and BMSCs transfected with scrambled siRNA (BMSCs+SS) with data given as mean +- SD ( n = 3). * p < 0.05 compared with the BMSC group. (C) Western blot analysis of RUNX2, OCN, and BSP from cell lysates of the BMSCs, BMSCs treated with HFC (BMSCs+HFC), TSG-6-siRNA-BMSCs treated with HFC (TSG-6-siRNA-BMSCs+HFC), and BMSCs transfected with scrambled siRNA (BMSCs+SS). (D-F) Densiometric analyses of western blot images. * p < 0.05, n = 3.