14-7139-81

antibody from Invitrogen Antibodies

Targeting: IL13 ALRH, BHR1, IL-13, MGC116786, MGC116788, MGC116789, P600

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Recommended by provider)

Blocking/Neutralizing (Recommended by provider)

Other assay (Recommended by provider)

Antibody Data

Product number

14-7139-81

Provider

Invitrogen Antibodies

Product name

IL-13 Monoclonal Antibody (PVM13-1), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The PVM13-1 antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8^+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression. Applications Reported: PVM13-1 has been reported for use in intracellular flow cytometric analysis, cytokine neutralization, and ELISA. (Fluorochrome conjugated PVM13-1 is recommended for use in intracellular flow cytometry.) (Please use Functional Grade purified PVM13-1, Product # 16-7139, in functional assays). Applications Tested: The PVM13-1 antibody has been tested as the capture antibody in a sandwich ELISA and ELISPOT assay for analysis of human interleukin-13 (IL-13) in combination with the biotinylated anti-human IL-13 polyclonal antibody (13-7138) antibody for detection and recombinant human IL-13 (14-8139) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

PVM13-1

Vial size

50 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

A molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22.

Chang HH, Liu GY, Dwivedi N, Sun B, Okamoto Y, Kinslow JD, Deane KD, Demoruelle MK, Norris JM, Thompson PR, Sparks JA, Rao DA, Karlson EW, Hung HC, Holers VM, Ho IC
JCI insight 2016 Oct 20;1(17):e90045

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COPA mutations impair ER-Golgi transport and cause hereditary autoimmune-mediated lung disease and arthritis.

Watkin LB, Jessen B, Wiszniewski W, Vece TJ, Jan M, Sha Y, Thamsen M, Santos-Cortez RL, Lee K, Gambin T, Forbes LR, Law CS, Stray-Pedersen A, Cheng MH, Mace EM, Anderson MS, Liu D, Tang LF, Nicholas SK, Nahmod K, Makedonas G, Canter DL, Kwok PY, Hicks J, Jones KD, Penney S, Jhangiani SN, Rosenblum MD, Dell SD, Waterfield MR, Papa FR, Muzny DM, Zaitlen N, Leal SM, Gonzaga-Jauregui C, Baylor-Hopkins Center for Mendelian Genomics, Boerwinkle E, Eissa NT, Gibbs RA, Lupski JR, Orange JS, Shum AK
Nature genetics 2015 Jun;47(6):654-60

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Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.

Price JV, Haddon DJ, Kemmer D, Delepine G, Mandelbaum G, Jarrell JA, Gupta R, Balboni I, Chakravarty EF, Sokolove J, Shum AK, Anderson MS, Cheng MH, Robinson WH, Browne SK, Holland SM, Baechler EC, Utz PJ
The Journal of clinical investigation 2013 Dec;123(12):5135-45

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Comparison of cytokine ELISpot assay formats for the detection of islet antigen autoreactive T cells. Report of the third immunology of diabetes society T-cell workshop.

Schloot NC, Meierhoff G, Karlsson Faresjö M, Ott P, Putnam A, Lehmann P, Gottlieb P, Roep BO, Peakman M, Tree T
Journal of autoimmunity 2003 Dec;21(4):365-76

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human PBMCs (300,000/well) in 100 µL complete RPMI medium were treated without (left) or with (right) Candida (100 µg/mL) for 24 hrs in an IL-13 ELISPOT assay.