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Learn500-P13-100UG
antibody from Invitrogen Antibodies
Targeting: IL13
ALRH, BHR1, IL-13, MGC116786, MGC116788, MGC116789, P600
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- ELISA [1]
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- Product number
- 500-P13-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-13 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MSPGPVPPST ALRELIEELV NITQNQKAPL CNGSMVWSIN LTAGMYCAAL ESLINVSGCS AIEKTQRMLS GFCPHKVSAG QFSSLHVRDT KIEVAQFVKD LLLHLKKLFR EGRFN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-13. Anti-Human IL-13-specific antibody was purified by affinity chromatography employing an immobilized Human IL-13 matrix. Sandwich ELISA: To detect Human IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-13 (500-P13Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-13. Western Blot: To detect Human IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-13 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Human Mesenchymal Stem Cell Secretome Driven T Cell Immunomodulation Is IL-10 Dependent.
Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.
Interleukin-13 and -4 expression in the central airways of smokers with chronic bronchitis.
IL-13 mRNA and immunoreactivity in allergen-induced rhinitis: comparison with IL-4 expression and modulation by topical glucocorticoid therapy.
Shephard MT, Merkhan MM, Forsyth NR
International journal of molecular sciences 2022 Nov 6;23(21)
International journal of molecular sciences 2022 Nov 6;23(21)
Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.
Chiu WK, Fann M, Weng NP
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7802-10
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):7802-10
Interleukin-13 and -4 expression in the central airways of smokers with chronic bronchitis.
Miotto D, Ruggieri MP, Boschetto P, Cavallesco G, Papi A, Bononi I, Piola C, Murer B, Fabbri LM, Mapp CE
The European respiratory journal 2003 Oct;22(4):602-8
The European respiratory journal 2003 Oct;22(4):602-8
IL-13 mRNA and immunoreactivity in allergen-induced rhinitis: comparison with IL-4 expression and modulation by topical glucocorticoid therapy.
Ghaffar O, Laberge S, Jacobson MR, Lowhagen O, Rak S, Durham SR, Hamid Q
American journal of respiratory cell and molecular biology 1997 Jul;17(1):17-24
American journal of respiratory cell and molecular biology 1997 Jul;17(1):17-24
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human IL-13 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-13 Polyclonal Antibody (Product # 500-P13-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-13 Polyclonal Antibody, Biotin (Product # 500-P13BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-13.
