Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 500826 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#500826, RRID:AB_2561679
- Product name
- Brilliant Violet 421™ anti-human IL-4
- Antibody type
- Monoclonal
- Antigen
- CHO-expressed, recombinant human IL-4
- Reactivity
- Human, Porcine
- Host
- Rat
- Conjugate
- Blue dye
- Isotype
- IgG
- Antibody clone number
- MP4-25D2
- Vial size
- 100 tests
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stimulated with PMA + ionomycin for 6 hours (in the presence of monensin), surface stained with CD3 FITC, fixed, permeabilized and then stained with IL-4 (clone MP4-25D2) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
- Conjugate
- Blue dye